By Wujing Xian
The box of biomedical engineering has greatly multiplied long ago 20 years, as mirrored within the elevated variety of bioengineering and biomaterials courses at universities. the expansion of this quarter has outpaced the advance of laboratory classes that permit scholars hands-on adventure, because the boundaries excited by developing multidisciplinary biomaterials laboratory classes are excessive. A Laboratory path in Biomaterials presents a instructing device finished in scope perspective.
Suitable for junior or senior point laboratory classes in biomaterials and bioengineering, this quantity trains scholars in laboratory talents, facts research, challenge fixing, and clinical writing. The textual content takes a multidisciplinary technique, integrating quite a few ideas that come with fabrics technological know-how, chemistry, biochemistry, molecular and cellphone biology, and engineering.
The writer provides versatile modules that let the coursework to be tailored to the wishes of other departments. each one module is equipped round a principal subject, resembling drug supply and common biomaterials, to augment scholar comprehension. This booklet offers step by step descriptions of lab tactics, reagents, apparatus, and knowledge processing guidance. additionally it is a sequence of thought-provoking questions and solutions following each one test, drawn from the author’s personal adventure in educating a biomaterials laboratory direction on the college of Illinois.
Timely in its insurance, a number of the experiments offered within the ebook are tailored from examine papers reflecting the development in a variety of disciplines of bioengineering and biomaterials technological know-how.
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Extra resources for A Laboratory Course in Biomaterials
Obtain the concentration for each sample individually. b. Use the concentrations of the duplicate samples to calculate the average and the standard error for each time point. c. Convert the concentration to weight with standard error for each time point. ) d. For each time point, calculate the cumulative weight of released BSA up to that time point. e. Convert the cumulative weight to cumulative percentage. Recall the amount of PLGA microspheres used for the controlled release and the drug load of the microspheres.
None. Disposable glass pipettes are OK, too. * Disclaimer: Commercial sources for reagents listed are used as examples only. The listing does not represent endorsement by the author. Similar or comparable reagents can be purchased from other commercial sources. See selection criteria. 40 A Laboratory Course in Biomaterials • Sonicator with microtip. Fisher Scientific Model 100 Sonic Dismembrator, Fisher cat. no. 15-338-53. Sel. : Power ~100 watts, and equipped with a fine tip probe (~1/8”). • Homogenizer.
Scenario 2: Later, you are trying to determine the encapsulation efficiency and the drug load, and where you are supposed to add 1000 µl of PBS to extract the BSA, your co-worker accidentally adds more than that 1000 µl so that neither of you knows how much PBS has been added. • Scenario 3: You are trying to determine BSA concentrations. 5 ml of Bradford reagent and 50 µl of BSA standard solution for each sample, but because of a mix-up, you have added only 5 µl of the BSA solution instead. • Scenario 4: Similar to Scenario 3, but instead of adding 50 µl BSA standard solution, you have added 55 µl.
A Laboratory Course in Biomaterials by Wujing Xian