By S Cortassa; et al
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Extra info for An introduction to metabolic and cellular engineering
In this respect, mathematical simulation of the desired process by attempting to incorporate into the model formulation as many variables as necessary to account for the known dynamical behavior will enhance the predictive power of the analysis and enlighten the decisions. g. cells, operate under homeodynamic conditions (see Chapter 5). These refer to the continuous motion of the system's dynamics, and its propensity for shifting between attractors at bifurcation points, based on its intrinsic dynamic properties (Fig.
Both the largest flux differences and the largest variations in enzyme levels were observed for the glycolytic enzymes. On the bases of: (i) the higher level of all glycolytic enzymes detected in low oxygen chemostat cultures, and (ii) the reduction only in the levels of hexokinase and pyruvate kinase, in low glucose chemostat cultures, Vriezen and van Dijken (1998) conclude that the down-regulation of glycolysis is only partially accomplished at the level of enzyme synthesis and flux modulation is primarily effected via concentrations of substrates, activators, and inhibitors (bottom layer according to Fig.
1994). g. actual reaction rates, enzyme kinetics, activation, inhibition (Fig. 6). Another timely topic for MCE, concerns the relationship between the different cellular processes, although these are frequently treated separately. g. , 2000). We will deal with this subject more thoroughly later (see Chapters 4 and 7). Transduction and Intracellular Signalling Cells exhibit many distinct signalling pathways that allow them to react to environmental stimuli. , 2000, for a review). g. the stress-sensing and the stress-transduction mechanisms.
An introduction to metabolic and cellular engineering by S Cortassa; et al